Monitoring of an Applied Beneficial Trichoderma Strain in Root-Associated Soil of Field-Grown Maize by MALDI-TOF MS
نویسندگان
چکیده
The persistence of beneficial microorganisms in the rhizosphere or surrounding soil following their application is a prerequisite for effective interaction with plant indigenous microbial communities respective habitats. goal study was to analyze establishment and applied Trichoderma harzianum (OMG16) strain maize root-associated depending on agricultural practice (soil management practice, N-fertilizer intensity) field experiment. A rapid identification inoculated OMG16 essential its monitoring. We used culture-based approach coupled matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis as part microbe consortium (BMc). isolated 428 fungal isolates from eight treatments Forty percent fungi equivalent 205 were identified Trichoderma, which 87% (=179 isolates) obtained fields BMc. Gene sequence showed high similarity MALDI-TOF MS-identified that confirming re-isolation added fungus. This highlighted use MS quick, cost-effective detection efficient monitoring tool microbial-based bioinoculants field.
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ژورنال
عنوان ژورنال: Microorganisms
سال: 2023
ISSN: ['2076-2607']
DOI: https://doi.org/10.3390/microorganisms11071655